h3k4me2 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc h3k4me2

596 interacts with LSD1 in EC Cells. (A). The molecular structure of 596. (B). Western blot detection of H3K4me1, <t>H3K4me2,</t> and H3K4me3 after 596(0,4,8,12,16 μM) treatment( n = 3). (C-D). After treating ISK and HEC1A cells with DMSO and 596 (12 μM) for 2 h, the thermal stability of LSD1 was detected by CETSA method. The quantitation (right) of western blot results. (E). In ISK and HEC1A cells, detect the proliferation of compound 596 after 48–96 h of different concentrations by CCK8. (F-G). GraphPad Prism 9 was used to calculate IC50 of 596 in ISK and HEC1A cells. (H). ISK cells and HEC1A cells were treated with different concentrations of 596 respectively, and the proliferation of the cells was detected by colony formation assay.

H3k4me2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3k4me2/Di-Methyl-Histone+H3+(Lys4)+Rabbit+mAb/pmc12914821-46-46-48
Average 95 stars, based on 183 article reviews

h3k4me2 - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway"

Article Title: The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway

Journal: Translational Oncology

doi: 10.1016/j.tranon.2026.102688

596 interacts with LSD1 in EC Cells. (A). The molecular structure of 596. (B). Western blot detection of H3K4me1, H3K4me2, and H3K4me3 after 596(0,4,8,12,16 μM) treatment( n = 3). (C-D). After treating ISK and HEC1A cells with DMSO and 596 (12 μM) for 2 h, the thermal stability of LSD1 was detected by CETSA method. The quantitation (right) of western blot results. (E). In ISK and HEC1A cells, detect the proliferation of compound 596 after 48–96 h of different concentrations by CCK8. (F-G). GraphPad Prism 9 was used to calculate IC50 of 596 in ISK and HEC1A cells. (H). ISK cells and HEC1A cells were treated with different concentrations of 596 respectively, and the proliferation of the cells was detected by colony formation assay.

Figure Legend Snippet: 596 interacts with LSD1 in EC Cells. (A). The molecular structure of 596. (B). Western blot detection of H3K4me1, H3K4me2, and H3K4me3 after 596(0,4,8,12,16 μM) treatment( n = 3). (C-D). After treating ISK and HEC1A cells with DMSO and 596 (12 μM) for 2 h, the thermal stability of LSD1 was detected by CETSA method. The quantitation (right) of western blot results. (E). In ISK and HEC1A cells, detect the proliferation of compound 596 after 48–96 h of different concentrations by CCK8. (F-G). GraphPad Prism 9 was used to calculate IC50 of 596 in ISK and HEC1A cells. (H). ISK cells and HEC1A cells were treated with different concentrations of 596 respectively, and the proliferation of the cells was detected by colony formation assay.

Techniques Used: Western Blot, Quantitation Assay, Colony Assay

Blocking Assay:

Article Title: Early-Life Arsenic Exposure Induces Histone H3K9 Methylation Causing PANoptosis and Neurodevelopmental Impairments in Offspring.
Article Snippet: Arsenic exposure is well-established to cause neurotoxicity, but its underlying molecular mechanisms remain unclear.. This study investigates the effects of early-life arsenic exposure on the nervous system of offspring.. Female mice were exposed to NaAsO2, and alterations in learning, memory, and hippocampal lesions in offspring were assessed.

Incubation:

Sonication:

Article Title: Tumoral RCOR2 promotes tumor development through dual epigenetic regulation of tumor plasticity and immunogenicity
Article Snippet: .. The chromatin was then sonicated and subjected to IP overnight in the presence of Protein G Dynabeads (Thermo Fisher Scientific) with antibodies against HA (Cell Signaling Technology, cat. no. 3724), HDAC1 (Bethyl Laboratories, cat. no. A300-713A), H3K4me2 (Cell Signaling Technology, cat. no. 9725), H4K16ac (Cell Signaling Technology, cat. no. 13534), or control rabbit IgG (Cell Signaling Technology, cat. no. 2729) at 4°C. ..

Article Title: Tumoral RCOR2 promotes tumor development through dual epigenetic regulation of tumor plasticity and immunogenicity
Article Snippet: .. The chromatin was then sonicated and subjected to IP overnight in the presence of Protein G Dynabeads (Thermo Fisher Scientific) with antibodies against HA (Cell Signaling Technology, catalog 3724), HDAC1 (Bethyl Laboratories, catalog A300-713A), H3K4me2 (Cell Signaling Technology, catalog 9725), and H4K16ac (Cell Signaling Technology, catalog 13534) or control rabbit IgG (Cell Signaling Technology, catalog 2729) at 4°C. ..

Control:

Article Title: Tumoral RCOR2 promotes tumor development through dual epigenetic regulation of tumor plasticity and immunogenicity
Article Snippet: .. The chromatin was then sonicated and subjected to IP overnight in the presence of Protein G Dynabeads (Thermo Fisher Scientific) with antibodies against HA (Cell Signaling Technology, catalog 3724), HDAC1 (Bethyl Laboratories, catalog A300-713A), H3K4me2 (Cell Signaling Technology, catalog 9725), and H4K16ac (Cell Signaling Technology, catalog 13534) or control rabbit IgG (Cell Signaling Technology, catalog 2729) at 4°C. ..

Cell Culture:

Article Title: The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway.
Article Snippet: .. The EC cells were cultured in DMEM medium(Viva cell, China) containing 10% fetal bovine serum (Viva cell, China) at 37◦C and 5% CO2.The main purchasing information was listed below:LSD1 (1:10,000, ab129195, Abcam, UK), H3(1:20,000, No.4499T,Cell Signaling Technology, CST, Boston, MA, USA), H3K4me1(1:2000, No.5326T, CST), H3K4me2(1:2000, No.9725S, CST), H3K4me3(1:2000, No. 9751T, CST), p62/SQSTM1(1:2000, No.5114T, CST), LC3B(1:2000, Cat# AF4650, Affinity Biosciences), Beclin-1(1:2000, Cat# AF5128, Affinity Biosciences), AKT(1:5000, YM8463, Immunoway, USK), P-AKT (Ser473)(1:2000, YM8304, Immunoway, USK), mTOR(1:5000,YM8208, Immunoway, USK), P-mTOR(Ser2448)(1:5000, YM8326, Immunoway, USK), S6(1:2000, YT4139, Immunoway, USK), P-S6(1:2000, No.F0198, Selleck.cn), p70S6K(1:2000, YT3559, Immunoway, USK), P-p70S6K (1:1000, No.F2907, Immunoway, USK), GAPDH(1:5000, Proteintech). .. Rapamycin, 3-MA was purchased from MCE and CC9001 was purchased from KKL Med Inc.

Article Title: The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway
Article Snippet: .. The EC cells were cultured in DMEM medium(Viva cell, China) containing 10% fetal bovine serum (Viva cell, China) at 37°C and 5% CO 2 .The main purchasing information was listed below:LSD1 (1:10,000, ab129195, Abcam, UK), H3(1:20,000, No.4499T,Cell Signaling Technology, CST, Boston, MA, USA), H3K4me1(1:2000, No.5326T, CST), H3K4me2(1:2000, No.9725S, CST), H3K4me3(1:2000, No. 9751T, CST), p62/SQSTM1(1:2000, No.5114T, CST), LC3B(1:2000, Cat# AF4650, Affinity Biosciences), Beclin-1(1:2000, Cat# AF5128, Affinity Biosciences), AKT(1:5000, YM8463, Immunoway, USK), P-AKT(Ser473)(1:2000, YM8304, Immunoway, USK), mTOR(1:5000,YM8208, Immunoway, USK), P-mTOR(Ser2448)(1:5000, YM8326, Immunoway, USK), S6(1:2000, YT4139, Immunoway, USK), P-S6(1:2000, No.F0198, Selleck.cn), p70S6K(1:2000, YT3559, Immunoway, USK), P-p70S6K(1:1000, No.F2907, Immunoway, USK), GAPDH(1:5000, Proteintech). .. Rapamycin, 3-MA was purchased from MCE and CC9001 was purchased from KKL Med Inc.

Western Blot:

Article Title: Epigenetic treatment of synaptic and behavioral deficits in Dyrk1a-mutant mice.
Article Snippet: .. Western blotting experiments for nuclear proteins were performed with antibodies against Histone 3 (cell signaling, 4499, 1:1000), H3K4me2 (cell signaling, 9725, 1:1000), and H3K9me2 (cell signaling, 4658, 1:1000). ..

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